Copine1 regulates neural stem cell functions during brain development

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Copine 1 (CPNE1) is a well-known phospholipid binding protein in plasma membrane of various cell types. In brain cells, CPNE1 is closely associated with AKT signaling pathway, which is important for neural stem cell (NSC) functions during brain development. Here, we investigated the role of CPNE1 in the regulation of brain NSC functions during brain development and determined its underlying mechanism. In this study, abundant expression of CPNE1 was observed in neural lineage cells including NSCs and immature neurons in human. With mouse brain tissues in various developmental stages, we found that CPNE1 expression was higher at early embryonic stages compared to postnatal and adult stages. To model developing brain in vitro, we used primary NSCs derived from mouse embryonic hippocampus. Our in vitro study shows decreased proliferation and multi-lineage differentiation potential in CPNE1 deficient NSCs. Finally, we found that the deficiency of CPNE1 downregulated mTOR signaling in embryonic NSCs. These data demonstrate that CPNE1 plays a key role in the regulation of NSC functions through the activation of AKT-mTOR signaling pathway during brain development.
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Issue Date
2018-01
Language
English
Article Type
Article
Keywords

PROGENITOR HIB5 CELLS; NEURONAL DIFFERENTIATION; CORTICAL DYSPLASIA; MOUSE-BRAIN; SOMATIC MUTATIONS; GLIOMA FORMATION; MTOR INHIBITION; EPILEPSY; DISEASE; MODEL

Citation

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.495, no.1, pp.168 - 173

ISSN
0006-291X
DOI
10.1016/j.bbrc.2017.10.167
URI
http://hdl.handle.net/10203/240571
Appears in Collection
BiS-Journal Papers(저널논문)
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