Multiplexed detection of foodborne pathogens from contaminated lettuces using a handheld multistep lateral flow assay device

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dc.contributor.authorShin, Joong Hoko
dc.contributor.authorHong, Jisooko
dc.contributor.authorGo, Hyeyunko
dc.contributor.authorPark, Juhwanko
dc.contributor.authorKong, Minsukko
dc.contributor.authorRyu, Sangryeolko
dc.contributor.authorKim, Kwang-Pyoko
dc.contributor.authorRoh, Eunjungko
dc.contributor.authorPark, Je-Kyunko
dc.date.accessioned2018-02-21T05:35:20Z-
dc.date.available2018-02-21T05:35:20Z-
dc.date.created2017-12-23-
dc.date.created2017-12-23-
dc.date.created2017-12-23-
dc.date.issued2018-01-
dc.identifier.citationJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.66, no.1, pp.290 - 297-
dc.identifier.issn0021-8561-
dc.identifier.urihttp://hdl.handle.net/10203/240110-
dc.description.abstractThis paper presents a handheld device that is capable of simplifying multistep assays to perform sensitive detection of foodborne pathogens. The device is capable of multiplexed detection of Escherichia coli (E. coli) O157:H7, Salmonella Typhimurium (S. Typhimurium), Staphylococcus aureus, and Bacillus cereus. The limit of detection for each bacterium was characterized, and then, the detection of bacteria from contaminated fresh lettuces was demonstrated for two representative foodborne pathogens. We employed a sample pretreatment protocol to recover and concentrate target bacteria from contaminated lettuces, which can detect 1.87 x 10(4) CFU of E. coli O157:H7 and 1.47 x 10(4) CFU of S. Typhimurium/1 g of lettuce without an enrichment process. Lastly, we demonstrated that the limit of detection can be reduced to 1 CFU of E. coli O157:H7 and 1 CFU of S. Typhimurium/1 g of lettuce by including a 6 h enrichment of contaminated lettuces in growth media before pretreatment.-
dc.languageEnglish-
dc.publisherAMER CHEMICAL SOC-
dc.titleMultiplexed detection of foodborne pathogens from contaminated lettuces using a handheld multistep lateral flow assay device-
dc.typeArticle-
dc.identifier.wosid000419999900034-
dc.identifier.scopusid2-s2.0-85040310534-
dc.type.rimsART-
dc.citation.volume66-
dc.citation.issue1-
dc.citation.beginningpage290-
dc.citation.endingpage297-
dc.citation.publicationnameJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY-
dc.identifier.doi10.1021/acs.jafc.7b03582-
dc.contributor.localauthorPark, Je-Kyun-
dc.contributor.nonIdAuthorHong, Jisoo-
dc.contributor.nonIdAuthorGo, Hyeyun-
dc.contributor.nonIdAuthorKong, Minsuk-
dc.contributor.nonIdAuthorRyu, Sangryeol-
dc.contributor.nonIdAuthorKim, Kwang-Pyo-
dc.contributor.nonIdAuthorRoh, Eunjung-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorcontaminated lettuces-
dc.subject.keywordAuthorfoodborne pathogens-
dc.subject.keywordAuthorhandheld device-
dc.subject.keywordAuthorlateral flow assay-
dc.subject.keywordAuthormultiplexed detection-
dc.subject.keywordPlus2-DIMENSIONAL PAPER NETWORK-
dc.subject.keywordPlusLISTERIA-MONOCYTOGENES-
dc.subject.keywordPlusRAPID DETECTION-
dc.subject.keywordPlusFRESH PRODUCE-
dc.subject.keywordPlusUNITED-STATES-
dc.subject.keywordPlusCHROMATOGRAPHY-
dc.subject.keywordPlusIMMUNOASSAY-
dc.subject.keywordPlusENHANCEMENT-
dc.subject.keywordPlusSENSITIVITY-
dc.subject.keywordPlusCHALLENGES-
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