Multiplexed detection of foodborne pathogens from contaminated lettuces using a handheld multistep lateral flow assay device

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This paper presents a handheld device that is capable of simplifying multistep assays to perform sensitive detection of foodborne pathogens. The device is capable of multiplexed detection of Escherichia coli (E. coli) O157:H7, Salmonella Typhimurium (S. Typhimurium), Staphylococcus aureus, and Bacillus cereus. The limit of detection for each bacterium was characterized, and then, the detection of bacteria from contaminated fresh lettuces was demonstrated for two representative foodborne pathogens. We employed a sample pretreatment protocol to recover and concentrate target bacteria from contaminated lettuces, which can detect 1.87 x 10(4) CFU of E. coli O157:H7 and 1.47 x 10(4) CFU of S. Typhimurium/1 g of lettuce without an enrichment process. Lastly, we demonstrated that the limit of detection can be reduced to 1 CFU of E. coli O157:H7 and 1 CFU of S. Typhimurium/1 g of lettuce by including a 6 h enrichment of contaminated lettuces in growth media before pretreatment.
Publisher
AMER CHEMICAL SOC
Issue Date
2018-01
Language
English
Article Type
Article
Keywords

2-DIMENSIONAL PAPER NETWORK; LISTERIA-MONOCYTOGENES; RAPID DETECTION; FRESH PRODUCE; UNITED-STATES; CHROMATOGRAPHY; IMMUNOASSAY; ENHANCEMENT; SENSITIVITY; CHALLENGES

Citation

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.66, no.1, pp.290 - 297

ISSN
0021-8561
DOI
10.1021/acs.jafc.7b03582
URI
http://hdl.handle.net/10203/240110
Appears in Collection
BiS-Journal Papers(저널논문)
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