DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, Jung Eun | ko |
dc.contributor.author | Park, Si Jae | ko |
dc.contributor.author | Kim, Won Jun | ko |
dc.contributor.author | Kim, Hyeong Jun | ko |
dc.contributor.author | Kim, Bumjoon J. | ko |
dc.contributor.author | Lee, Hyuk | ko |
dc.contributor.author | Shin, Jihoon | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.date.accessioned | 2018-02-21T05:08:13Z | - |
dc.date.available | 2018-02-21T05:08:13Z | - |
dc.date.created | 2018-01-22 | - |
dc.date.created | 2018-01-22 | - |
dc.date.created | 2018-01-22 | - |
dc.date.created | 2018-01-22 | - |
dc.date.created | 2018-01-22 | - |
dc.date.issued | 2018-01 | - |
dc.identifier.citation | NATURE COMMUNICATIONS, v.9 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | http://hdl.handle.net/10203/239914 | - |
dc.description.abstract | Aromatic polyesters are widely used plastics currently produced from petroleum. Here we engineer Escherichia coli strains for the production of aromatic polyesters from glucose by one-step fermentation. When the Clostridium difficile isocaprenoyl-CoA: 2-hydroxyisocaproate CoA-transferase (HadA) and evolved polyhydroxyalkanoate (PHA) synthase genes are overexpressed in a D-phenyllactate-producing strain, poly(52.3 mol% 3-hydroxybutyrate (3HB)-co-47.7 mol% D-phenyllactate) can be produced from glucose and sodium 3HB. Also, various poly(3HB-co-D-phenyllactate) polymers having 11.0, 15.8, 20.0, 70.8, and 84.5 mol% of D-phenyllactate are produced from glucose as a sole carbon source by additional expression of Ralstonia eutropha beta-ketothiolase (phaA) and reductase (phaB) genes. Fed-batch culture of this engineered strain produces 13.9 g l(-1) of poly(61.9 mol% 3HB-co-38.1 mol% D-phenyllactate). Furthermore, different aromatic polyesters containing D-mandelate and D-3-hydroxy-3-phenylpropionate are produced from glucose when feeding the corresponding monomers. The engineered bacterial system will be useful for one-step fermentative production of aromatic polyesters from renewable resources. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | One-step fermentative production of aromatic polyesters from glucose by metabolically engineered Escherichia coli strains | - |
dc.type | Article | - |
dc.identifier.wosid | 000419446600007 | - |
dc.identifier.scopusid | 2-s2.0-85042799027 | - |
dc.type.rims | ART | - |
dc.citation.volume | 9 | - |
dc.citation.publicationname | NATURE COMMUNICATIONS | - |
dc.identifier.doi | 10.1038/s41467-017-02498-w | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Bumjoon J. | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.contributor.nonIdAuthor | Park, Si Jae | - |
dc.contributor.nonIdAuthor | Lee, Hyuk | - |
dc.contributor.nonIdAuthor | Shin, Jihoon | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | POLYLACTIC ACID | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | POLYHYDROXYALKANOATES | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | BIOPLASTICS | - |
dc.subject.keywordPlus | COPOLYMERS | - |
dc.subject.keywordPlus | COHESION | - |
dc.subject.keywordPlus | SYNTHASE | - |
dc.subject.keywordPlus | PLASTICS | - |
dc.subject.keywordPlus | PATHWAY | - |
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