Biophysical characterization of the basic cluster in the transcription repression domain of human MeCP2 with AT-rich DNA

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MeCP2 is a chromatin associated protein which is highly expressed in brain and relevant with Rett syndrome (RTT). There are AT-hook motifs in MeCP2 which can bind with AT-rich DNA, suggesting a role in chromatin binding. Here, we report the identification and characterization of another AT-rich DNA binding motif (residues 295 to 313) from the C-terminal transcription repression domain of MeCP2 by nuclear magnetic resonance (NMR) and isothermal calorimetry (ITC). This motif shows a micromolar affinity to AT-rich DNA, and it binds to the minor groove of DNA like AT-hook motifs. Together with the previous studies, our results provide an insight into a critical role of this motif in chromatin structure and function.
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Issue Date
2018-01
Language
English
Article Type
Article
Keywords

BINDING PROTEIN MECP2; RETT-SYNDROME; METHYL-CPG; HOOK MOTIFS; COMPLEX; CHROMATIN; RELAXATION; DISORDERS; SEQUENCE

Citation

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.495, no.1, pp.145 - 150

ISSN
0006-291X
DOI
10.1016/j.bbrc.2017.10.169
URI
http://hdl.handle.net/10203/238157
Appears in Collection
BS-Journal Papers(저널논문)
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