Disclosed herein are an α1/CaV3.1 double knockout mouse or α1−/−; Emx1-Cre mouse with enhanced essential tremor and a screening method of therapeutic agents for essential tremor by using the same. The α1/CaV3.1 double knockout mouse or α1−/−; Emx1-Cre mouse of the present invention may be usefully used for development of therapeutic agents for essential tremor because the mouse exhibits essential tremor strong and evident enough to be visually confirmed, compared to an α1 knockout mouse.