A bi-slot tree based tag identification method in RFID systems includes (a) transmitting an inquiry of (n 1) bits to each tag at the reader; (b) deciding whether the inquiry of (n 1) bits are identical with first (n 1) bits of each tag ID when receiving the inquiry of (n 1) bits at each tag within a readable range of the reader; (c) transmitting each tag ID by one bit from (n+1)th bit to last bit thereof to the reader through different time slots based on nth bit value of each tag ID at the tags whose first (n 1) bits are identical with the inquiry of (n 1) bits; and (d) at the reader, checking whether there is a collision among the tag IDs received from the tags and identifying each tag ID of the tags based on the check result.