Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process

This study examined nine expired industrial Corynebacterium glutamicum strains with high lysine producing capability for enhanced production of 5-AVA. C. glutamicum KCTC 1857 exhibiting the highest lysine production was transformed with either original Pseudomonas putida davBA genes, encoding the 5-AVA biosynthesis pathway, or C. glutamicum codon-optimized davBA genes. C. glutamicum KCTC 1857 expressing the original genes had superior cell viability and 5-AVA production capability compared to the other strain. This strain produced 39.93 g/L of 5-AVA, which is the highest titer reported to date in fed-batch fermentation from glucose. Indeed, Miscanthus hydrolysate solution prepared from a novel process, comprising pretreatment, hydrolysis, purification, and concentration, was used as feedstock for 5-AVA production. A total of 12.51 g/L 5-AVA was produced from the Miscanthus hydrolysate; this value is 34.7% higher than that obtained from glucose in batch fermentation.
Publisher
ELSEVIER SCI LTD
Issue Date
2017-12
Language
English
Keywords

SUBSEQUENT ENZYMATIC-HYDROLYSIS; HIGH-LEVEL CONVERSION; ESCHERICHIA-COLI; L-LYSINE; CHEMICALS; BIOMASS; HYDROTHERMOLYSIS; EXPRESSION; CADAVERINE; GLUTARATE

Citation

BIORESOURCE TECHNOLOGY, v.245, pp.1692 - 1700

ISSN
0960-8524
DOI
10.1016/j.biortech.2017.05.131
URI
http://hdl.handle.net/10203/226814
Appears in Collection
CBE-Journal Papers(저널논문)
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