Optimization and Scale-Up of Succinic Acid Production by Mannheimia succiniciproducens LPK7

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The effects of culture conditions on succinic acid production and its possible scale-up have been studied. Mannheimia succiniciproducens LPK7, engineered for enhanced production of succinic acid and reduced by-product secretion, was used for the experiments. Mannheimia succiniciproducens LPK7 is a knock-out strain of wild type deficient in the IdhA, pflB, and pta-ackA genes, and is derived from Mannheimia succiniciproducens MBEL55E. Process optimization of factors including optimal temperature, pH, carbon source, and nitrogen source was performed to enhance the production of succinic acid in flasks. To observe scale-up effects, batch fermentation was carried out at various working volumes. At a working volume of 7.0 l, the final succinic acid concentration and yield were 15.4 g/l and 0.86 g/g. This result shows similar amount of succinic acid obtained in lab-scale fermentation, and it is possible to scale up to larger fermentors without major problems.
Publisher
KOREAN SOC MICROBIOLOGY BIOTECHNOLOGY
Issue Date
2009-02
Language
English
Article Type
Article
Keywords

ENGINEERED ESCHERICHIA-COLI; ANAEROBIOSPIRILLUM-SUCCINICIPRODUCENS; FERMENTATION; BACTERIUM; RUMEN

Citation

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.19, no.2, pp.167 - 171

ISSN
1017-7825
DOI
10.4014/jmb.0807.447
URI
http://hdl.handle.net/10203/22638
Appears in Collection
CBE-Journal Papers(저널논문)
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