Thiomaleimide Functionalization for Selective Biological Fluorescence Detection of Peroxynitrite as Tested in HeLa and RAW 264.7 Cells

Cited 20 time in webofscience Cited 0 time in scopus
  • Hit : 227
  • Download : 0
The role of fluorescent molecules in diagnosis, treatment as well as in biomedical research has great current medicinal significance and is the focus of concentrated effort across the scientific research spectrum. Related research continues to reveal new practical sensing systems that bear enhanced features for interfacing of substituted molecules with biological systems. As part of an effort to better understand chalcogenide systems, a new dithiomaleimide BODIPY (BDP-NGM) probe has been designed, synthesized and characterized. The fluorescence of BDP-NGM was quenched by the incorporation of [3,4-bis(phenylthio)] on the maleimide-4-phenyl moiety which is, in turn, placed at the meso-position of the BODIPY system. The probe shows a turn-on fluorescence response upon reaction with ONOO-; mass spectral evidence reveals peaks in agreement with products involving oxidation of the sulfur groups to sulfone groups. An about 18.0-fold emission intensity enhancement was found. By comparison, the emission signal from another ROS/RNS, superoxide, gave a modest turn on signal (approximate to 5.0-fold). The reaction is complete within 10 min, judging from the monitoring of the turn-on fluorescence process; the detection limit was found to be 0.4 mu m. BDP-NGM can be used for the detection of ONOO- under both acidic and basic conditions. Live cell imaging showed that the current probe can be used for the selective detection of ONOO- in living systems.
Publisher
WILEY-V C H VERLAG GMBH
Issue Date
2017-08
Language
English
Article Type
Article
Keywords

ANTITUMOR AGENT AA1; LIVING CELLS; NITRIC-OXIDE; TURN-ON; PHOTODYNAMIC THERAPY; MUSCLE FOODS; CANCER-CELLS; LUMINOL CHEMILUMINESCENCE; HYPOCHLOROUS ACID; BODIPYS SYNTHESIS

Citation

CHEMISTRY-AN ASIAN JOURNAL, v.12, no.15, pp.1927 - 1934

ISSN
1861-4728
DOI
10.1002/asia.201700527
URI
http://hdl.handle.net/10203/226312
Appears in Collection
CH-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 20 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0