Measuring an exact amount of remaining cryogenic liquid propellant under microgravity condition is one of the important issuesof rocket vehicle. A Pressure-Volume-Temperature (PVT) gauging method is attractive due to its minimal additional hardware andsimple gauging process. In this paper, PVT gauging method using liquid nitrogen is investigated under microgravity condition withparabolic flight. A 9.2 litre metal cryogenic liquid storage tank containing approximately 30% of liquid nitrogen is pressurized byambient temperature helium gas. During microgravity condition, the inside of the liquid tank becomes near-isothermal conditionwithin 1 K difference indicated by 6 silicon diode sensors vertically distributed in the middle of the liquid tank. Helium injectionwith higher mass flow rate after 10 seconds of the waiting time results in successful measurements of helium partial pressure in thetank. Average liquid volume measurement error is within 11% of the whole liquid tank volume and standard deviation of errors is11.9. As a result, the applicability of PVT gauging method to liquid propellant stored in space is proven with good measurementaccuracy.