Optogenetic protein clustering through fluorescent protein tagging and extension of CRY2

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Protein homo-oligomerization is an important molecular mechanism in many biological processes. Therefore, the ability to control protein homo-oligomerization allows the manipulation and interrogation of numerous cellular events. To achieve this, cryptochrome 2 (CRY2) from Arabidopsis thaliana has been recently utilized for blue light-dependent spatiotemporal control of protein homo-oligomerization. However, limited knowledge on molecular characteristics of CRY2 obscures its widespread applications. Here, we identify important determinants for efficient cryptochrome 2 clustering and introduce a new CRY2 module, named "CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins by employing diverse fluorescent proteins and an extremely short peptide. Furthermore, we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools. Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2017-06
Language
English
Article Type
Article
Keywords

MAMMALIAN-CELLS; CRYPTOCHROME 2; BLUE-LIGHT; LIVE CELLS; OLIGOMERIZATION; TOOL

Citation

NATURE COMMUNICATIONS, v.8

ISSN
2041-1723
DOI
10.1038/s41467-017-00060-2
URI
http://hdl.handle.net/10203/224872
Appears in Collection
BS-Journal Papers(저널논문)
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