DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kong, Minsuk | ko |
dc.contributor.author | Shin, Joong Ho | ko |
dc.contributor.author | Heu, Sunggi | ko |
dc.contributor.author | Park, Je-Kyun | ko |
dc.contributor.author | Ryu, Sangryeol | ko |
dc.date.accessioned | 2017-07-18T05:42:09Z | - |
dc.date.available | 2017-07-18T05:42:09Z | - |
dc.date.created | 2017-07-01 | - |
dc.date.created | 2017-07-01 | - |
dc.date.created | 2017-07-01 | - |
dc.date.issued | 2017-10 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, v.96, pp.173 - 177 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/10203/224769 | - |
dc.description.abstract | The development of a cost-effective and efficient bacterial detection assay is essential for diagnostic fields, particularly in resource-poor settings. Although antibodies have been widely used for bacterial capture, the production of soluble antibodies is still expensive and time-consuming. Here, we developed a nitrocellulose-based lateral flow assay using cell wall binding domains (CBDs) from phage as a recognition element and colloidal gold nanoparticles as a colorimetric signal for the detection of a model pathogenic bacterium, Bacillus cereus (B. cereus). To improve conjugation efficiency and detection sensitivity, cysteine-glutathione-S-transferase-tagged CBDs and maltose-binding protein-tagged CBDs were produced in Escherichia coli (E. coli) and incorporated in our assays. The sensitivity of the strip to detect B. cereus was 1x10(4) CFU/mL and the overall assay time was 20 min. The assay showed superior results compared to the antibody-based approach, and did not show any significant cross-reactivity. This proof of concept study indicates that the lateral flow assay using engineered CBDs hold considerable promise as simple, rapid, and cost-effective biosensors for whole cell detection. | - |
dc.language | English | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Lateral flow assay-based bacterial detection using engineered cell wall binding domains of a phage endolysin | - |
dc.type | Article | - |
dc.identifier.wosid | 000403419700024 | - |
dc.identifier.scopusid | 2-s2.0-85018381939 | - |
dc.type.rims | ART | - |
dc.citation.volume | 96 | - |
dc.citation.beginningpage | 173 | - |
dc.citation.endingpage | 177 | - |
dc.citation.publicationname | BIOSENSORS & BIOELECTRONICS | - |
dc.identifier.doi | 10.1016/j.bios.2017.05.010 | - |
dc.contributor.localauthor | Park, Je-Kyun | - |
dc.contributor.nonIdAuthor | Kong, Minsuk | - |
dc.contributor.nonIdAuthor | Heu, Sunggi | - |
dc.contributor.nonIdAuthor | Ryu, Sangryeol | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Bacillus cereus | - |
dc.subject.keywordAuthor | Bacteriophage | - |
dc.subject.keywordAuthor | Biosensor | - |
dc.subject.keywordAuthor | Cell wall binding domain | - |
dc.subject.keywordAuthor | Paper strip | - |
dc.subject.keywordPlus | BACILLUS-CEREUS | - |
dc.subject.keywordPlus | LISTERIA-MONOCYTOGENES | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | ANTIBODIES | - |
dc.subject.keywordPlus | EXPRESSION | - |
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