Revealing genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles under osmotic stress in Escherichia coli K-12 MG1655

Cited 4 time in webofscience Cited 0 time in scopus
  • Hit : 181
  • Download : 113
A transcription factor (TF), OmpR, plays a critical role in transcriptional regulation of the osmotic stress response in bacteria. Here, we reveal a genome-scale OmpR regulon in Escherichia coli K-12 MG1655. Integrative data analysis reveals that a total of 37 genes in 24 transcription units (TUs) belong to OmpR regulon. Among them, 26 genes show more than two-fold changes in expression level in an OmpR knock-out strain. Specifically, we find that: 1) OmpR regulates mostly membrane-located gene products involved in diverse fundamental biological processes, such as narU (encoding nitrate/nitrite transporter), ompX (encoding outer membrane protein X), and nuoN (encoding NADH: ubiquinone oxidoreductase); 2) by investigating co-regulation of entire sets of genes regulated by other stressresponse TFs, stresses are surprisingly independently regulated among each other; and, 3) a detailed investigation of the physiological roles of the newly discovered OmpR regulon genes reveals that activation of narU represents a novel strategy to significantly improve osmotic stress tolerance of E. coli. Thus, the genome-scale approach to elucidating regulons comprehensively identifies regulated genes and leads to fundamental discoveries related to stress responses.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2017-05
Language
English
Article Type
Article
Keywords

CHIP-SEQ DATA; NEGATIVE REGULATION; OXIDATIVE STRESS; GENE; PROTEIN; OSMOLARITY; EXPRESSION; NETWORK; SYSTEM; RNA

Citation

SCIENTIFIC REPORTS, v.7

ISSN
2045-2322
DOI
10.1038/s41598-017-02110-7
URI
http://hdl.handle.net/10203/224076
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
000401614900039.pdf(2.26 MB)Download
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 4 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0