We present an impedance monitoring microchamber array for discriminating normal and cancer cells, the top layer of which is covered to form a stable contact between the cells and the electrodes in each microchamber. Compared with devices in previous works, the present device is capable of maintaining long-term cell-to-electrode contact, simply by loading the cells on the electrodes placed in each microchamber and covering the layer, thereby achieving frequency-dependent impedance analysis without additional apparatus. By comparing the impedance response of two human lung cancer cell lines with a normal lung cell line, we verified that the cancer cells are clearly distinguishable against the normal cells by showing both lower resistance (241 vs 271 k Omega) and capacitance (3.13 vs 7.01 nF) in the frequency range of 200 Hz to 2 MHz. Simplicity of cell loading and the capability for determining characteristics of the cancer cells were demonstrated using the device.