A new s-adenosylhomocysteine hydrolase-linked method for adenosine detection based on DNA-templated fluorescent Cu/Ag nanoclusters

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We herein describe a novel fluorescent method for the rapid and selective detection of adenosine by utilizing DNA-templated Cu/Ag nanoclusters (NCs) and employing s-adenosylhomocysteine hydrolase (SAHH). SAHH is allowed to promote hydrolysis reaction of s-adenosylhomocysteine (SAH) and consequently produces homocysteine, which would quench the fluorescence signal from DNA-templated Cu/Ag nanoclusters employed as a signaling probe in this study. On the other hand, adenosine significantly inhibits the hydrolysis reaction and prevent the formation of homocysteine. Consequently, highly enhanced fluorescence signal from DNA-Cu/Ag NCs is retained, which could be used to identify the presence of adenosine. By employing this design principle, adenosine was sensitively detected down to 19 nM with high specificity over other adenosine analogs such as AMP, ADP, ATP, cAMP, guanosine, cytidine, and urine. Finally, the diagnostic capability of this method was successfully verified by reliably detecting adenosine present in a real human serum sample. (C) 2016 Elsevier B.V. All rights reserved.
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Issue Date
2017-07
Language
English
Article Type
Article; Proceedings Paper
Keywords

LABEL-FREE; AMPLIFIED DETECTION; IONS; APTAMER; CANCER; APTASENSOR; PROBES; CELLS; FLUID

Citation

BIOSENSORS & BIOELECTRONICS, v.93, pp.330 - 334

ISSN
0956-5663
DOI
10.1016/j.bios.2016.08.058
URI
http://hdl.handle.net/10203/223760
Appears in Collection
CBE-Journal Papers(저널논문)
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