An integrated rotary microfluidic system with DNA extraction, loop-mediated isothermal amplification, and lateral flow strip based detection for point-of-care pathogen diagnostics

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Point-of-care (POC) molecular diagnostics plays a pivotal role for the prevention and treatment of infectious diseases. In spite of recent advancement in microfluidic based POC devices, there are still rooms for development to realize rapid, automatic and cost-effective sample-to-result genetic analysis. In this study, we propose an integrated rotary microfluidic system that is capable of performing glass microbead based DNA extraction, loop mediated isothermal amplification (LAMP), and colorimetric lateral flow strip based detection in a sequential manner with an optimized microfluidic design and a rotational speed control. Rotation direction dependent coriolis force and siphon valving structures enable us to perform the fluidic control and metering, and the use of the lateral flow strip as a detection method renders all the analytical processes for nucleic acid test simplified and integrated without the need of expensive instruments or human intervention. As a proof of concept for point-of-care DNA diagnostics, we identified the food-borne bacterial pathogen which was contaminated in water or milk. Not only monoplex Salmonella Typhimurium but also multiplex Salmonella Typhimurium and Vibrio parahaemolyticus were analysed on the integrated rotary genetic analysis micro system with a limit of detection of 50 CFU in 80 min. In addition, three multiple samples were simultaneously analysed on a single device. The sample-to-result capability of the proposed microdevice provides great usefulness in the fields of clinical diagnostics, food safety and environment monitoring.
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Issue Date
2017-05
Language
English
Article Type
Article
Keywords

INFLUENZA-A VIRUS; NUCLEIC-ACID EXTRACTION; IMMUNOCHROMATOGRAPHIC STRIP; SAMPLE PREPARATION; CHIP; MICRODEVICE; PCR; IDENTIFICATION; PURIFICATION; COMBINATION

Citation

BIOSENSORS & BIOELECTRONICS, v.91, pp.334 - 340

ISSN
0956-5663
DOI
10.1016/j.bios.2016.11.063
URI
http://hdl.handle.net/10203/223575
Appears in Collection
CBE-Journal Papers(저널논문)
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