Vertical order of DPPC multilayer enhanced by cholesterol-induced ripple-to-liquid ordered (LO) phase transition: Synchrotron X-ray reflectivity study

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We investigated the effect of cholesterol on the vertical order of spin-coated supported dipalmitoyl-phosphatidylcholine (DPPC) multilayers by using synchrotron X-ray reflectivity with customized X-ray liquid cell. As increasing mol% of cholesterol up to 10%, the lamellar spacing of the DPPC multilayer increases, implying that cholesterol induces a tilt-to-untilt transition. The sharpening of X-ray intensity upon cholesterol binding, reflected in the FWHM plot, suggests that cholesterol enhances the vertical ordering of the DPPC multilayer. In addition, fluorescence microscopy experiment confirms a gel-to liquid ordered (LO) phase transition between 5 and 7% cholesterol. As a result, in low cholesterol regime, the DPPC multilayer is not in a simple gel phase but in a ripple phase where lipids are vertically disordered due to the differently tilted chains which leads to undulations. Therefore, we conclude that the cholesterol-induced ripple-to-LO phase transition enhances the vertical order of the DPPC multilayer. While most of works on membrane structure and cholesterol effect have focused on the physical properties of lipid membrane in lateral direction, this paper suggested a new physical aspect of studying vertical structures of membrane. (C) 2016 Elsevier B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE BV
Issue Date
2017-03
Language
English
Article Type
Article
Keywords

DIFFERENTIAL SCANNING CALORIMETRY; NUCLEAR-MAGNETIC-RESONANCE; SUPPORTED LIPID-BILAYERS; DIPALMITOYLPHOSPHATIDYLCHOLINE BILAYERS; CELL-MEMBRANE; DOMAIN FORMATION; MODEL SYSTEMS; RAFTS; MONOLAYERS; DISEASE

Citation

CURRENT APPLIED PHYSICS, v.17, no.3, pp.392 - 397

ISSN
1567-1739
DOI
10.1016/j.cap.2016.12.022
URI
http://hdl.handle.net/10203/223265
Appears in Collection
BiS-Journal Papers(저널논문)
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