DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Seo, Tae Seok | - |
dc.contributor.advisor | 서태석 | - |
dc.contributor.author | Lee, Dohwan | - |
dc.contributor.author | 이도환 | - |
dc.date.accessioned | 2017-03-29T02:37:59Z | - |
dc.date.available | 2017-03-29T02:37:59Z | - |
dc.date.issued | 2016 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=649654&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/221739 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 전기및전자공학부, 2016.2 ,[iii, 26 p. :] | - |
dc.description.abstract | We have developed an integrated direct loop-mediated isothermal amplification (Direct LAMP) mi-crodevice incorporated with an immunochromatographic strip (ICS) to identify bacteria contaminated in real samples. The Direct LAMP is a novel isothermal DNA amplification technique which does not require thermal cycling steps as well as any sample preparation steps such as cell lysis and DNA extraction for amplifying specific target genes. In addition, the resultant amplicons were colorimetrically detected on the ICS, thereby enabling the entire genetic analysis process to be simplified. The two functional units (Direct LAMP and ICS) were integrated on a single device without use of the tedious and complicated microvalve and tubing systems. The utilization of a slidable plate allows us to manipulate the fluidic control in the microchannels manually and the sequential operation of the Direct LAMP and ICS detection could be performed by switching the slidable plate to each functional unit. Thus, the combination of the direct isothermal amplification without any sample preparation and thermal cycling steps, the ICS based amplicon detection by naked eyes, and the slidable plate to eliminate the microvalves in the integrated microdevice would be an ideal platform for point-of-care DNA diaganotics. On the integrated Direct LAMP-ICS microdevice, we could analyze Staphylococcus aureus (S. aureus) and Escherichia coli O157:H7 (E. coli O157:H7) contaminated in human whole blood or milk at a single-cell level within 1 h. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | direct loop-mediated isothermal amplification | - |
dc.subject | no sample pretreatment | - |
dc.subject | valveless | - |
dc.subject | colorimetric detection | - |
dc.subject | integrated microdevice | - |
dc.subject | 무전처리 고리매개 등온증폭 | - |
dc.subject | 무밸브형 통합 마이크로 디바이스 | - |
dc.subject | 슬라이더블 챔버 | - |
dc.subject | 면역크로마토그래피용 스트립 | - |
dc.subject | 박테리아 검출 | - |
dc.title | (An) integrated valveless microdevice for rapid and multiplex detection of bacteria in human whole blood and milk without a sample preparation step | - |
dc.title.alternative | 전처리 과정 없이 박테리아의 신속한 다중 검출을 위한 무밸브형 통합 마이크로 디바이스 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :전기및전자공학부, | - |
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