(A) study on utilization of lipid extracted aurantiochytrium sp. biomass as nitrogen source탈 지질 aurantiochytrium sp. 바이오매스의 질소원으로의 활용에 관한 연구

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In order to solve environmental problems caused by an increased use of fossil fuel, microalgal biodiesel has been considered as an alternative energy source. However, the main challenge for microalgal biodiesel is to improve economic feasibility of the production. Many have studied on the possible solutions, particularly on using a low-cost medium for microalgal cultivation or co-producing various value-added products. In this study, a combination of both solutions was investigated. Lipid extracted algae (LEA) was used as a nutrient source to cultivate Aurantiochytrium sp. KRS101, and this was used to produce biodiesel and docosahexaenoic acid (DHA) which is a value-added product. LEA were hydrolyzed with various acids (sulfuric acid, hydrochloric acid, nitric acid) in different conditions (temperature, time, catalyst concentration) in order to be utilized as a nutrient source. Due to a high content of protein and a low content of carbohydrate in Aurantiochytrium sp. KRS101 LEA, it was appropriate to use it as a nitrogen source rather than a carbon source. LEA hydrolysate also showed a high concentration of phosphate, which was enough to replace a phosphorous source. The highest amino acids yield was 1767 mg/L when treated with 2N hydrochloric acid at $120^\circ C$. Among amino acids, gamma-aminobutyric acid (GABA), which is receiving attention as a material for nylon 4, occupied large portion. For the cultivation, 20 g/L of LEA treated with 2N hydrochloric acid at $120^\circ C$ by an autoclave was used. Cells cultivated with LEA hydrolysate and supplementary yeast extract showed similar growth (10.0 g/L) with a control (10.6 g/L). It also showed similar fatty acid methyl esters (FAMEs) and DHA contents (46%, 13.3%, dry cell weight basis) with a control (50.2%, 13.3%, dry cell weight basis). These results showed that hydrolysate of Aurantiochytrium sp. KRS101 LEA can be utilized as a low-cost nutrient, replacing the yeast extract and monopotassium phosphate in the cultivation of Aurantiochytrium sp. KRS101 used for production of biodiesel and DHA.
Advisors
Chang, Yong Keunresearcher장용근researcher
Description
한국과학기술원 :생명화학공학과,
Publisher
한국과학기술원
Issue Date
2016
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2016.2 ,[iv, 49 p. :]

Keywords

Microalgae; Biodiesel; Lipid extracted algae (LEA); Aurantiochytrium sp.; Docosahexaenoic acid (DHA); gamma-aminobutyric acid (GABA); 미세조류; 바이오연료; 탈 지질 바이오매스; DHA; 감마아미노부티릭산

URI
http://hdl.handle.net/10203/221519
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=649477&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
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