DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Sung Mi | ko |
dc.contributor.author | Jeoung, Sae Chae | ko |
dc.contributor.author | Song, Ji-Young | ko |
dc.contributor.author | Song, Ji-Joon | ko |
dc.contributor.author | Park, Youn-Il | ko |
dc.date.accessioned | 2017-03-28T04:14:05Z | - |
dc.date.available | 2017-03-28T04:14:05Z | - |
dc.date.created | 2017-02-14 | - |
dc.date.created | 2017-02-14 | - |
dc.date.issued | 2017-01 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v.7 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/10203/220725 | - |
dc.description.abstract | Cyanobacteriochromes (CBCRs) are a subfamily of phytochrome photoreceptors found exclusively in photosynthetic cyanobacteria. Four CBCRs containing a second Cys in the insert region (insert-Cys) have been identified from the nonheterocystous cyanobacterium Microcoleus B353 (Mbr3854g4 and Mbl3738g2) and the nitrogen fixing, heterocystous cyanobacterium Nostoc punctiforme (NpF2164g3 and NpR1597g2). These insert-Cys CBCRs can sense light in the near-UV to orange range, but key residues responsible for tuning their colour sensitivity have not been reported. In the present study, near-UV/Green (UG) photosensors Mbr3854g4 (UG1) and Mbl3738g2 (UG2) were chosen for further spectroscopic analysis of their spectral sensitivity and tuning. Consistent with most dual-Cys CBCRs, both UGs formed a second thioether linkage to the phycocyanobilin (PCB) chromophore via the insert-Cys. This bond is subject to breakage and relinkage during forward and reverse photoconversions. Variations in residues equivalent to Phe that are in close contact with the PCB chromophore D-ring in canonical red/green CBCRs are responsible for tuning the light absorption peaks of both dark and photoproducts. This is the first time these key residues that govern light absorption in insert-Cys family CBCRs have been identified and characterised. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | SP PCC 6803 | - |
dc.subject | GAF DOMAIN | - |
dc.subject | THERMOSYNECHOCOCCUS-ELONGATUS | - |
dc.subject | REVERSIBLE PHOTOCONVERSION | - |
dc.subject | DXCF-CYANOBACTERIOCHROMES | - |
dc.subject | NEGATIVE PHOTOTAXIS | - |
dc.subject | NOSTOC-PUNCTIFORME | - |
dc.subject | CELL-AGGREGATION | - |
dc.subject | LIGHT | - |
dc.subject | PHYTOCHROME | - |
dc.title | Hydrophobic Residues near the Bilin Chromophore-Binding Pocket Modulate Spectral Tuning of Insert-Cys Subfamily Cyanobacteriochromes | - |
dc.type | Article | - |
dc.identifier.wosid | 000391939400001 | - |
dc.identifier.scopusid | 2-s2.0-85009832203 | - |
dc.type.rims | ART | - |
dc.citation.volume | 7 | - |
dc.citation.publicationname | SCIENTIFIC REPORTS | - |
dc.identifier.doi | 10.1038/srep40576 | - |
dc.contributor.localauthor | Song, Ji-Joon | - |
dc.contributor.nonIdAuthor | Cho, Sung Mi | - |
dc.contributor.nonIdAuthor | Jeoung, Sae Chae | - |
dc.contributor.nonIdAuthor | Song, Ji-Young | - |
dc.contributor.nonIdAuthor | Park, Youn-Il | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SP PCC 6803 | - |
dc.subject.keywordPlus | GAF DOMAIN | - |
dc.subject.keywordPlus | THERMOSYNECHOCOCCUS-ELONGATUS | - |
dc.subject.keywordPlus | REVERSIBLE PHOTOCONVERSION | - |
dc.subject.keywordPlus | DXCF-CYANOBACTERIOCHROMES | - |
dc.subject.keywordPlus | NEGATIVE PHOTOTAXIS | - |
dc.subject.keywordPlus | NOSTOC-PUNCTIFORME | - |
dc.subject.keywordPlus | CELL-AGGREGATION | - |
dc.subject.keywordPlus | LIGHT | - |
dc.subject.keywordPlus | PHYTOCHROME | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.