Zein-alginate based oral drug delivery systems: Protection and release of therapeutic proteins

Cited 17 time in webofscience Cited 0 time in scopus
  • Hit : 362
  • Download : 0
Reactive oxygen species (ROS) play an important role in the development of inflammatory bowel diseases. Superoxide dismutase (SOD) has a great therapeutic potential by scavenging superoxide that is one of ROS; however, in vivo application is limited especially when it is orally administered. SOD is easily degraded in vivo by the harsh conditions of gastrointestinal tract. Here, we design a zein-alginate based oral drug delivery system that protects SOD from the harsh conditions of gastrointestinal tract and releases it in the environment of the small intestine. SOD is encapsulated in zein-alginate nanoparticles (ZAN) via a phase separation method. We demonstrate that ZAN protect SOD from the harsh conditions of the stomach or small intestine condition. ZAN (200: 40) at the weight ratio of 200 mg zein to 40 mg of alginate releases SOD in a pH dependent manner, and it releases 90.8 +/- 1.2% of encapsulated SOD at pH 7.4 in 2 h, while only 11.4 +/- 0.4% of SOD was released at pH 1.3. The encapsulation efficiency of SOD in ZAN (200: 40) was 62.1 +/- 2.0%. SOD in ZAN (200: 40) reduced the intracellular ROS level and it saved 88.9 +/- 7.5% of Caco-2 cells from the toxic superoxide in 4 hours. Based on the results, zein-alginate based oral drug delivery systems will have numerous applications to drugs that are easily degradable in the harsh conditions of gastrointestinal tract. (C) 2016 Elsevier B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE BV
Issue Date
2016-12
Language
English
Article Type
Article
Keywords

INFLAMMATORY-BOWEL-DISEASE; SUPEROXIDE-DISMUTASE; OXIDATIVE STRESS; SIGNAL-TRANSDUCTION; STORAGE PROTEINS; CELIAC-DISEASE; PATHOGENESIS; MACROPHAGES; MECHANISMS

Citation

INTERNATIONAL JOURNAL OF PHARMACEUTICS, v.515, no.1-2, pp.300 - 306

ISSN
0378-5173
DOI
10.1016/j.ijpharm.2016.10.023
URI
http://hdl.handle.net/10203/220148
Appears in Collection
CBE-Journal Papers(저널논문)BiS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 17 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0