A late-lineage murine neutrophil precursor population exhibits dynamic changes during demand-adapted granulopoiesis

Cited 8 time in webofscience Cited 0 time in scopus
  • Hit : 545
  • Download : 182
Homeostasis of neutrophils-the blood cells that respond first to infection and tissue injury-is critical for the regulation of immune responses and regulated through granulopoiesis, a multistage process by which neutrophils differentiate from hematopoietic stem cells. Granulopoiesis is a highly dynamic process and altered in certain clinical conditions, such as pathologic and iatrogenic neutropenia, described as demand-adapted granulopoiesis. The regulation of granulopoiesis under stress is not completely understood because studies of granulopoiesis dynamics have been hampered by technical limitations in defining neutrophil precursors. Here, we define a population of neutrophil precursor cells in the bone marrow with unprecedented purity, characterized by the lineage(-)CD11b(+)Ly6G(lo)Ly6B(int)CD115(-), which we call NeuPs (Neutrophil Precursors). We demonstrated that NeuPs differentiate into mature and functional neutrophils both in vitro and in vivo. By analyzing the gene expression profiles of NeuPs, we also identified NeuP stage-specific genes and characterized patterns of gene regulation throughout granulopoiesis. Importantly, we found that NeuPs have the potential to proliferate, but the proliferation decreased in multiple different hematopoietic stress settings, indicating that proliferating NeuPs are poised at a critical step to regulate granulopoiesis. Our findings will facilitate understanding how the hematopoietic system maintains homeostasis and copes with the demands of granulopoiesis.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2017-01
Language
English
Article Type
Article
Keywords

COLONY-STIMULATING FACTOR; BINDING-PROTEIN EPSILON; BONE-MARROW-CELLS; EMERGENCY GRANULOPOIESIS; G-CSF; IN-VIVO; DEFICIENT MICE; LISTERIA-MONOCYTOGENES; TRANSCRIPTION FACTORS; DENDRITIC CELLS

Citation

SCIENTIFIC REPORTS, v.7

ISSN
2045-2322
DOI
10.1038/srep39804
URI
http://hdl.handle.net/10203/220118
Appears in Collection
BiS-Journal Papers(저널논문)BS-Journal Papers(저널논문)
Files in This Item
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 8 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0