Digital DNA detection based on a compact optofluidic laser with ultra-low sample consumption

Cited 16 time in webofscience Cited 0 time in scopus
  • Hit : 488
  • Download : 0
DNA lasers self-amplify optical signals from a DNA analyte as well as thermodynamic differences between sequences, allowing quasi-digital DNA detection. However, these systems have drawbacks, such as relatively large sample consumption and complicated dye labelling. Moreover, although the lasing signal can detect the target DNA, it is superimposed on an unintended fluorescence background, which persists for non-target DNA samples as well. From an optical point of view, it is thus not truly digital detection and requires spectral analysis to identify the target. In this work, we propose and demonstrate an optofluidic laser that has a single layer of DNA molecules as the gain material. A target DNA produces intensive laser emission comparable to existing DNA lasers, while any unnecessary fluorescence background is successfully suppressed. As a result, the target DNA can be detected with a single laser pulse, in a truly digital manner. Since the DNA molecules cover only a single layer on the surface of the laser microcavity, the DNA sample consumption is a few orders of magnitude lower than that of existing DNA lasers. Furthermore, the DNA molecules are stained by simply immersing the microcavity in the intercalating dye solution, and thus the proposed DNA laser is free of any complex dye-labelling process prior to analysis.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2016
Language
English
Article Type
Article
Keywords

MELTING ANALYSIS; HUMAN-DISEASE; METHYLATION ANALYSIS; MICROCAVITY LASER; RING-RESONATOR; OLIGONUCLEOTIDE; SINGLE; MICROARRAYS; GENOME; GAIN

Citation

LAB ON A CHIP, v.16, no.24, pp.4770 - 4776

ISSN
1473-0197
DOI
10.1039/c6lc01258b
URI
http://hdl.handle.net/10203/218339
Appears in Collection
NT-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 16 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0