DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Myung-Chul | ko |
dc.contributor.author | Chung, Peter J | ko |
dc.contributor.author | Song, Chaeyeon | ko |
dc.contributor.author | Miller, Herbert P | ko |
dc.contributor.author | Kiris, E | ko |
dc.contributor.author | Lid, Youli | ko |
dc.contributor.author | Wilson, Leslie | ko |
dc.contributor.author | Feinstein, Stuart C | ko |
dc.contributor.author | Safinya, Cyrus R | ko |
dc.date.accessioned | 2017-01-12T07:12:37Z | - |
dc.date.available | 2017-01-12T07:12:37Z | - |
dc.date.created | 2016-12-27 | - |
dc.date.created | 2016-12-27 | - |
dc.date.created | 2016-12-27 | - |
dc.date.issued | 2017-01 | - |
dc.identifier.citation | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v.1861, no.1, pp.3456 - 3463 | - |
dc.identifier.issn | 0304-4165 | - |
dc.identifier.uri | http://hdl.handle.net/10203/218286 | - |
dc.description.abstract | Background: Microtubules (MTs) are protein nanotubes comprised of straight protofilaments (PFs), head to tail assemblies of alpha beta-tubulin heterodimers. Previously, it was shown that Tau, a microtubule-associated protein (MAP) localized to neuronal axons, regulates the average number of PFs in microtubules with increasing inner radius <R-in(MT)> observed for increasing Tau/tubulin-dimer molar ratio Phi(Tau) at paclitaxel/tubulin-dimer molar ratio Lambda(ptxl) = 1/1. Methods: We report a synchrotron SAXS and TEM study of the phase behavior of microtubules as a function of varying concentrations of paclitaxel (1/32 <= Lambda(ptxl) <= 1/4) and Tau (human isoform 3RS, 0 <= Phi(3Rs) <= 1/2) at room temperature. Results: Tau and paclitaxel have opposing regulatory effects on microtubule bundling architectures and microtubule diameter. Surprisingly and in contrast to previous results at Air = 1/1 where microtubule bundles are absent, in the lower paclitaxel concentration regime (Lambda(ptxl) <= 1/4), we observe both microtubule doublets and triplets with increasing Tau. Furthermore, increasing paditaxel concentration (up to Lambda(ptxl) = 1/1) slightly decreased the average microtubule diameter (by similar to 1 PF) while increasing Tau concentration (up to Phi(3RS) = 1/2) significantly increased the diameter (by similar to 2-3 PFs). Conclusions: The suppression of Tau-mediated microtubule bundling with increasing paclitaxel is consistent with paditaxel seeding more, but shorter, microtubules by rapidly exhausting tubulin available for polymerization. Microtubule bundles require the aggregate Tau-Tau attractions along the microtubule length to overcome individual microtubule thermal energies disrupting bundles. General significance: Investigating MAP-mediated interactions between microtubules (as it relates to in vivo behavior) requires the elimination or minimization of paclitaxel. (C) 2016 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | X-RAY-SCATTERING | - |
dc.subject | FORMATION IN-VITRO | - |
dc.subject | THERMAL FLUCTUATIONS | - |
dc.subject | DYNAMIC INSTABILITY | - |
dc.subject | PROTEIN-TAU | - |
dc.subject | TUBULIN | - |
dc.subject | TAXOL | - |
dc.subject | PROTOFILAMENTS | - |
dc.subject | BINDING | - |
dc.subject | DOMAINS | - |
dc.title | Paclitaxel suppresses Tau-mediated microtubule bundling in a concentration-dependent manner | - |
dc.type | Article | - |
dc.identifier.wosid | 000390736300050 | - |
dc.identifier.scopusid | 2-s2.0-84994528965 | - |
dc.type.rims | ART | - |
dc.citation.volume | 1861 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 3456 | - |
dc.citation.endingpage | 3463 | - |
dc.citation.publicationname | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | - |
dc.identifier.doi | 10.1016/j.bbagen.2016.09.011 | - |
dc.contributor.localauthor | Choi, Myung-Chul | - |
dc.contributor.nonIdAuthor | Chung, Peter J | - |
dc.contributor.nonIdAuthor | Song, Chaeyeon | - |
dc.contributor.nonIdAuthor | Miller, Herbert P | - |
dc.contributor.nonIdAuthor | Kiris, E | - |
dc.contributor.nonIdAuthor | Lid, Youli | - |
dc.contributor.nonIdAuthor | Wilson, Leslie | - |
dc.contributor.nonIdAuthor | Feinstein, Stuart C | - |
dc.contributor.nonIdAuthor | Safinya, Cyrus R | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Tau | - |
dc.subject.keywordAuthor | Paclitaxel | - |
dc.subject.keywordAuthor | Microtubule | - |
dc.subject.keywordAuthor | Small-angle X-ray scattering | - |
dc.subject.keywordPlus | X-RAY-SCATTERING | - |
dc.subject.keywordPlus | FORMATION IN-VITRO | - |
dc.subject.keywordPlus | THERMAL FLUCTUATIONS | - |
dc.subject.keywordPlus | DYNAMIC INSTABILITY | - |
dc.subject.keywordPlus | PROTEIN-TAU | - |
dc.subject.keywordPlus | TUBULIN | - |
dc.subject.keywordPlus | TAXOL | - |
dc.subject.keywordPlus | PROTOFILAMENTS | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | DOMAINS | - |
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