Enhanced Fluorescence Turn-on Imaging of Hypochlorous Acid in Living Immune and Cancer Cells

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Two closely related phenyl selenyl based boron-di-pyrromethene (BODIPY) turn-on fluorescent probes for the detection of hypochlorous acid (HOCl) were synthesized for studies in chemical biology; emission intensity is modulated by a photoinduced electron-transfer (PET) process. Probe 2 intrinsically shows a negligible background signal; however, after reaction with HOCl, chemical oxidation of selenium forecloses the PET process, which evokes a significant increase in fluorescence intensity. The fluorescence intensity of probes 1 and 2 with HOCl involves an similar to 18 and similar to 50-fold enhancement compared with the respective responses from other reactive oxygen/nitrogen species (ROS/RNS) and low detection limits (30.9 nM for 1 and 4.5 nM for 2). Both probes show a very fast response with HOCl; emission intensity reached a maximum within 1 s. These probes show high selectivity for HOCl, as confirmed by confocal microscopy imaging when testing with RAW264.7 and MCF-7 cells
Publisher
WILEY-V C H VERLAG GMBH
Issue Date
2016-07
Language
English
Article Type
Article
Keywords

HUMAN ATHEROSCLEROTIC LESIONS; INDUCED HOCL PRODUCTION; SELECTIVE DETECTION; RUTHENIUM(II) COMPLEX; NEUTROPHIL PHAGOSOME; OXIDATIVE STRESS; REACTIVE OXYGEN; BODIPY DYES; LIVE CELLS; PROBE

Citation

CHEMISTRY-A EUROPEAN JOURNAL, v.22, no.28, pp.9642 - 9648

ISSN
0947-6539
DOI
10.1002/chem.201601270
URI
http://hdl.handle.net/10203/212913
Appears in Collection
BS-Journal Papers(저널논문)CH-Journal Papers(저널논문)
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