On-chip generation of monodisperse giant unilamellar lipid vesicles containing quantum dots

Abstract

Monodispersed lipid vesicles have been used as a drug delivery vehicle and a biochemical reactor. To generate monodispersed lipid vesicles in the nano-to micrometer size range, an extrusion step should be included in conventional hand-shaking method of lipid vesicle synthesis. In addition, lipid vesicles as a drug carrier still need to be improved to effectively encapsulate concentrated biomolecules such as cells, proteins, and target drugs. To overcome these limitations, this paper reports a new microfluidic platform for continuous synthesis of small-sized (similar to 10 mu m) giant unilamellar vesicles (GUVs) containing quantum dots (QDs) as a nanosized model drug. To generate GUVs, we introduced an additional cross-flow to break vesicles into small size. 1,2 - dimyristoyl-sn-glycero - 3 - phosphocholine (DMPC) in an octanol-chloroform mixture was used in the construction of self-assembled membrane. Consequently, we have successfully demonstrated the fabrication of monodispersed GUVs with 7-12 mu m diameter containing QDs. The proposed synthesis method of cell-sized GUVs would be highly desirable for applications such as multipurpose drug encapsulation and delivery