Molecular Architecture of Yeast Chromatin Assembly Factor 1

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Chromatin Assembly Complex 1 (CAF-1) is a major histone chaperone involved in deposition of histone H3 and H4 into nucleosome. CAF-1 is composed of three subunits; p150, p60 and p48 for human and Cac1, Cac2 and Cac3 for yeast. Despite of its central role in chromatin formation, structural features of the full CAF-1 in complex with histones and other chaperones have not been well characterized. Here, we dissect molecular architecture of yeast CAF-1 (yCAF-1) by cross-linking mass spectrometry (XL-MS) and negative stain single-particle electron microscopy (EM). Our work revealed that Cac1, the largest subunit of yCAF-1, might serve as a major histone binding platform linking Cac2 and Cac3. In addition, EM analysis showed that yCAF-1 adopts a bilobal shape and Cac1 connecting Cac2 and Cac3 to generate a platform for binding histones. This study provides the first structural glimpse of the full CAF-1 complex and a structural framework to understand histone chaperoning processes
Publisher
NATURE PUBLISHING GROUP
Issue Date
2016-05
Language
English
Article Type
Article
Keywords

NEWLY SYNTHESIZED HISTONES; DNA-REPLICATION; STRUCTURAL BASIS; METHYLTRANSFERASE ACTIVITY; H4; ASF1; ACETYLATION; CAF-1; H3; DEPOSITION

Citation

SCIENTIFIC REPORTS, v.6

ISSN
2045-2322
DOI
10.1038/srep26702
URI
http://hdl.handle.net/10203/209731
Appears in Collection
BS-Journal Papers(저널논문)
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