Label-free electrochemical detection of botulinum neurotoxin type E based on its enzymatic activity using interdigitated electrodes

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We report a simple label-free electrochemical method of detecting low concentrations of botulinum neurotoxin type E light chain (BoNT/E LC) based on its peptide cleavage activity. Dual-mode cyclic voltammetry was employed to observe changes in the redox signal of ferri-/ferro-cyanide on interdigitated microelectrodes, whose surfaces were covered by peptides designed from synaptosomal-associated protein 25 to be cleaved by BoNT/E LC. With the introduction of BoNT/E LC, the redox signal showed a time-dependent increase due to cleavage of the immobilized peptide molecules. In addition to the increased redox signal intensity, its time-dependence can be considered as a strong evidence of BoNT/E sensing, since the time-dependent increase can only result from the enzymatic activity of BoNT/E LC. Using this method, BoNT/E LC, at concentrations as low as 5 pg/ml, was readily measurable with only an hour of incubation. (C) 2016 AIP Publishing LLC
Publisher
AMER INST PHYSICS
Issue Date
2016-02
Language
English
Article Type
Article
Keywords

GOLD NANOPARTICLES; TOXIN; IMMUNOSENSOR; DIAGNOSIS; SURFACES; ASSAY

Citation

APPLIED PHYSICS LETTERS, v.108, no.9

ISSN
0003-6951
DOI
10.1063/1.4942800
URI
http://hdl.handle.net/10203/209365
Appears in Collection
BS-Journal Papers(저널논문)
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