Synaptic compartmentalization by micropatterned masking of a surface adhesive cue in cultured neurons

Cited 5 time in webofscience Cited 0 time in scopus
  • Hit : 214
  • Download : 0
Functions of neuronal circuit are fundamentally modulated by its quality and quantity of connections. Assessment of synapse, the basic unit for a neuronal connection, is labor-intensive and time-consuming in conventional culture systems, due to the small size and the spatially random distribution. In the present study, we propose a novel 'synapse compartmentalization' culture system, in which synapses are concentrated at controlled locations. We fabricated a negative dot array pattern by coating the entire surface with poly-l-lysine (PLL) and subsequent microcontact printing of 1) substrates which mask positive charge of NI (Fc, BSA and laminin), or 2) a chemorepulsive protein (Semaphorin 3F-Fc). By combination of physical and biological features of these repulsive substrates, functional synapses were robustly concentrated in the PLL-coated dots. This synapse compartmentalization chip can be combined with the various high-throughput assay formats based on the synaptic morphology and function. Therefore, this quantifiable and controllable dot array pattern by microcontact printing will be potential useful for bio-chip platforms for the high-density assays used in synapse-related neurobiological studies. (C) 2016 Elsevier Ltd. All rights reserved.
Publisher
ELSEVIER SCI LTD
Issue Date
2016-06
Language
English
Article Type
Article
Keywords

DENDRITE GROWTH; IN-VITRO; GUIDANCE; SPECIFICATION; MATURATION; GRADIENTS; MOLECULES; MIGRATION; OUTGROWTH; PROTEINS

Citation

BIOMATERIALS, v.92, pp.46 - 56

ISSN
0142-9612
DOI
10.1016/j.biomaterials.2016.03.027
URI
http://hdl.handle.net/10203/209201
Appears in Collection
BiS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 5 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0