Laser spectroscopic characterization and quantification of uranium(VI) under fluorescence quenching by Fe(II)

Abstract

Spectroscopic characteristics of aqueous uranium(VI) (U(VI)) (0.01-1 mu Ie) and its quantification procedure in the presence of Fe(II) (a parts per thousand currency sign0.09 mM) have been developed at pH 7 using TRLFS. The measured fluorescence properties of U(VI) in the phosphate solutions showed the co-presence of UO2HPO4 (aq) and The fluorescence signals of each species were remarkably quenched following dominantly static quenching process via the formation of non-fluorescent complexes. An empirical equation correcting the quenched fluorescence intensity was developed and the corrected values of quenched U(VI) intensity showed a consistency (a parts per thousand yen93.39 +/- A 2.27 %) with the measured uranium concentration by ICP-MS.

We firstly confirmed that aqueous U(VI) quantification by TRLFS can be significantly interrupted by Fe(II) quenching via dominantly a static quenching process. The novel analytical procedure for correcting quenched fluorescence intensity (concentration) of U(VI) was developed based on the relationship of the quenched intensity and Fe(II) concentration at neutral pH.