Optimal production of poly-gamma-glutamic acid by metabolically engineered Escherichia coli

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Metabolically-engineered Escherichia coli strains were developed by cloning poly-gamma-glutamic acid (gamma-PGA) biosynthesis genes, consisting of pgsB, pgsC and pgsA, from Bacillus subtilis The metabolic and regulatory pathways of gamma-PGA biosynthesis in E. coli were analyzed by DNA microarray. The inducible trc promoter and a constitutive promoter (P-HCE) derived from the D-amino acid aminotransferase (D-AAT) gene of Geobacillus toebii were employed. The constitutive HCE promoter was more efficient than inducible trc promoter for the expression of gamma-PGA biosynthesis genes. DNA microarray analysis showed that the expression levels of several NtrC family genes, glnA, glnK, glnG, yhdX, yhdY, yhdZ, amtB, nac, argT and cbl were up-regulated and sucA, B, C, D genes were down-regulated. When (NH4)(2)SO4 was added at 40 g/l into the feeding solution, the final gamma-PGA concentration reached 3.7 g/l in the fed-batch culture of recombinant E. coli/pCOpgs.
Publisher
SPRINGER
Issue Date
2006-08
Language
English
Article Type
Article
Keywords

BACILLUS-LICHENIFORMIS; CULTURE

Citation

BIOTECHNOLOGY LETTERS, v.28, pp.1241 - 1246

ISSN
0141-5492
DOI
10.1007/s10529-006-9080-0
URI
http://hdl.handle.net/10203/20872
Appears in Collection
CBE-Journal Papers(저널논문)
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