Molecular mass of poly[(R)-3-hydroxybutyric acid] produced in a recombinant Escherichia coli

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Poly[(R)-3-hydroxybutyric acid] (PHB) was produced at 37 degrees C by a recombinant Escherichia coli harboring the Alcaligenes eutrophus biosynthesis phb-CAB genes in Luria-Bertani media containing glucose at 10-30 g/l at different pH values and the time-dependent changes in the molecular mass of PHB were studied. PHB polymers accumulated within cells while glucose was present in the medium. The number-average molecular mass of PHB decreased with time during the course of PHB accumulation, and the values for PHB were markedly dependent on the cultivation conditions of the E. coli, ranging from 0.5 MDa to 20 MDa. Under specific conditions (pH 6.0), E. coli produced PHB with an extremely high molecular mass (20 MDa). It has been suggested that a chain-transfer agent is generated in E. coli cells during the accumulation of PHB.
Publisher
SPRINGER VERLAG
Issue Date
1997-02
Language
English
Article Type
Article
Citation

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.47, no.2, pp.140 - 143

ISSN
0175-7598
URI
http://hdl.handle.net/10203/20764
Appears in Collection
CBE-Journal Papers(저널논문)
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