Ultrafast sonochemical synthesis of proteininorganic nanoflowers

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We developed a simple but efficient method to synthesize protein-inorganic hybrid nanostructures with a flower-like shape (nanoflowers), which relies on sonication to facilitate the synthesis of the nanoflowers. With this technique, we synthesized nanoflowers containing laccase as a model protein and copper phosphate within 5 minutes at room temperature. The resulting laccase nanoflowers yielded greatly enhanced activity, stability, and reusability, and their usefulness was successfully demonstrated by applying them in the colorimetric detection of epinephrine. The strategy developed could be used to rapidly synthesize nanoflowers for various applications in biosensor and enzyme catalysis and would expand the utilization of nanoflowers in diverse fields of biotechnology.
Publisher
DOVE MEDICAL PRESS LTD
Issue Date
2015-08
Language
English
Article Type
Article
Keywords

INORGANIC HYBRID NANOFLOWERS; MAGNETIC NANOPARTICLES; PEROXIDASE MIMETICS; FACILE SYNTHESIS; ENZYME; SENSOR; IMMOBILIZATION; EPINEPHRINE; OXIDASE; PHENOL

Citation

INTERNATIONAL JOURNAL OF NANOMEDICINE, v.10, pp.137 - 142

ISSN
1178-2013
DOI
10.2147/IJN.S90274
URI
http://hdl.handle.net/10203/207551
Appears in Collection
CBE-Journal Papers(저널논문)
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