Emergent genetic oscillations in a synthetic microbial consortium

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A challenge of synthetic biology is the creation of cooperative microbial systems that exhibit population-level behaviors. Such systems use cellular signaling mechanisms to regulate gene expression across multiple cell types. We describe the construction of a synthetic microbial consortium consisting of two distinct cell types-an "activator" strain and a "repressor" strain. These strains produced two orthogonal cell-signaling molecules that regulate gene expression within a synthetic circuit spanning both strains. The two strains generated emergent, population-level oscillations only when cultured together. Certain network topologies of the two-strain circuit were better at maintaining robust oscillations than others. The ability to program population-level dynamics through the genetic engineering of multiple cooperative strains points the way toward engineering complex synthetic tissues and organs with multiple cell types.
Publisher
AMER ASSOC ADVANCEMENT SCIENCE
Issue Date
2015-08
Language
English
Article Type
Article
Citation

SCIENCE, v.349, no.6251, pp.986 - 989

ISSN
0036-8075
DOI
10.1126/science.aaa3794
URI
http://hdl.handle.net/10203/207013
Appears in Collection
MA-Journal Papers(저널논문)
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