A novel electroporation system for efficient molecular delivery into Chlamydomonas reinhardtii with a 3-dimensional microelectrode

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Electroporation is one of the most widely used transfection methods because of its high efficiency and convenience among the various transfection methods. Previous micro-electroporation systems have some drawbacks such as limitations in height and design, time-consuming and an expensive fabrication process due to technical constraints. This study fabricates a three dimensional microelectrode using the 3D printing technique. The interdigitated microstructure consisting of poly lactic acid was injected by a 3D printer and coated with silver and aluminum with a series of dip-coatings. With the same strength of electric field (V cm(-1)), a higher efficiency for molecular delivery and a higher cellular viability are achieved with the microelectrode than with a standard cuvette. In addition, this study investigates chemicophysical changes such as Joule heating and dissolved metal during electroporation and showed the micro-electroporation system had less chemicophysical changes. It was concluded that the proposed micro-electroporation system will contribute to genetic engineering as a promising delivery tool, and this combination of 3D printing and electroporation has many potential applications for diverse designs or systems.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2015-11
Language
English
Article Type
Article
Keywords

MICROFLUIDIC ELECTROPORATION; NUCLEAR TRANSFORMATION; CHEMICAL-SYNTHESIS; GENE-THERAPY; STEM-CELLS; IN-VIVO; TRANSFECTION; DEVICE; NANOPARTICLES; REACTIONWARE

Citation

SCIENTIFIC REPORTS, v.5

ISSN
2045-2322
DOI
10.1038/srep15835
URI
http://hdl.handle.net/10203/205186
Appears in Collection
CBE-Journal Papers(저널논문)
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