Genome-wide microRNA screening reveals that the evolutionary conserved miR-9a regulates body growth by targeting sNPFR1/NPYR

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MicroRNAs (miRNAs) regulate many physiological processes including body growth. Insulin/ IGF signalling is the primary regulator of animal body growth, but the extent to which miRNAs act in insulin-producing cells (IPCs) is unclear. Here we generate a UAS-miRNA library of Drosophila stocks and perform a genetic screen to identify miRNAs whose overexpression in the IPCs inhibits body growth in Drosophila. Through this screen, we identify miR-9a as an evolutionarily conserved regulator of insulin signalling and body growth. IPC-specific miR-9a overexpression reduces insulin signalling and body size. Of the predicted targets of miR-9a, we find that loss of miR-9a enhances the level of sNPFR1. We show via an in vitro binding assay that miR-9a binds to sNPFR1 mRNA in insect cells and to the mammalian orthologue NPY2R in rat insulinoma cells. These findings indicate that the conserved miR-9a regulates body growth by controlling sNPFR1/NPYR-mediated modulation of insulin signalling.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2015-07
Language
English
Article Type
Article
Keywords

INSULIN-LIKE PEPTIDES; NEUROPEPTIDE-Y; DROSOPHILA; EXPRESSION; RECEPTOR; CELLS

Citation

NATURE COMMUNICATIONS, v.6

ISSN
2041-1723
DOI
10.1038/ncomms8693
URI
http://hdl.handle.net/10203/204011
Appears in Collection
BS-Journal Papers(저널논문)
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