Effect of post-induction nutrient feeding strategies on the production of bioadhesive protein in Escherichia coli

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The effect of post-induction nutrient feeding strategies on the production of bioadhesive protein using an IPTG inducible expression system in Escherichia coil was investigated. Cells were cultured in an exponential fed-batch mode to the OD600 of ca. 100 (48 gDCW/L) prior to induction. Six different post-induction nutrient feeding strategies (pH-stat, exponential, constant and linear change in feeding rate with three different slopes) were then applied, and bioadhesive protein production was examined. It was found that post-induction cell growth was independent of nutrient feeding rate. However, bioadhesive protein production was significantly affected by post-induction feeding strategies. Linearly changing post-induction feeding rate with a suitable slope allowed production of bioadhesive protein up to 5.3 g/L, which was higher than that obtained by the other post-induction feeding strategies. (C) 1998 John Wiley & Sons, Inc.
Publisher
JOHN WILEY SONS INC
Issue Date
1998-11
Language
English
Article Type
Article
Citation

BIOTECHNOLOGY AND BIOENGINEERING, v.60, no.3, pp.271 - 276

ISSN
0006-3592
URI
http://hdl.handle.net/10203/20339
Appears in Collection
CBE-Journal Papers(저널논문)
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