Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partner

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A novel strategy for micropatterning proteins on the surface of polyhydroxyalkanoate (PHA) biopolymer by microcontact printing (mu CP) is described. The substrate binding domain (SBD) of the Pseudomonas stutzeri PHA depolymerase was used as a fusion partner for specifically immobilizing proteins on PHA substrate. Enhanced green fluorescent protein (EGFP) and red fluorescent protein (RFP) fused to the SBD could be specifically immobilized on the micropatterns of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). Laser scanning confocal microscopic studies suggested that two fusion proteins were micropatterned in their functionally active forms. Also, antibody binding assay by surface plasmon resonance suggested that protein-protein interaction studies could be carried out using this system. (c) 2005 Wiley Periodicals, Inc.
Publisher
JOHN WILEY & SONS INC
Issue Date
2005-10
Language
English
Article Type
Article
Citation

BIOTECHNOLOGY AND BIOENGINEERING, v.92, no.2, pp.160 - 165

ISSN
0006-3592
DOI
10.1002/bit.20581
URI
http://hdl.handle.net/10203/20218
Appears in Collection
CH-Journal Papers(저널논문)CBE-Journal Papers(저널논문)
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