Cyclic ADP Ribose-Dependent Ca2+ Release by Group I Metabotropic Glutamate Receptors in Acutely Dissociated Rat Hippocampal Neurons

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Group I metabotropic glutamate receptors (group I mGluRs; mGluR1 and mGluR5) exert diverse effects on neuronal and synaptic functions, many of which are regulated by intracellular Ca2+. In this study, we characterized the cellular mechanisms underlying Ca2+ mobilization induced by (RS)-3,5-dihydroxyphenylglycine (DHPG; a specific group I mGluR agonist) in the somata of acutely dissociated rat hippocampal neurons using microfluorometry. We found that DHPG activates mGluR5 to mobilize intracellular Ca2+ from ryanodine-sensitive stores via cyclic adenosine diphosphate ribose (cADPR), while the PLC/IP3 signaling pathway was not involved in Ca2+ mobilization. The application of glutamate, which depolarized the membrane potential by 28.5 +/- 4.9 mV (n = 4), led to transient Ca2+ mobilization by mGluR5 and Ca2+ influx through L-type Ca2+ channels. We found no evidence that mGluR5-mediated Ca2+ release and Ca2+ influx through L-type Ca2+ channels interact to generate supralinear Ca2+ transients. Our study provides novel insights into the mechanisms of intracellular Ca2+ mobilization by mGluR5 in the somata of hippocampal neurons.
Publisher
PUBLIC LIBRARY SCIENCE
Issue Date
2011-10
Language
English
Article Type
Article
Keywords

CA1 PYRAMIDAL NEURONS; LONG-TERM DEPRESSION; BACKPROPAGATING ACTION-POTENTIALS; SEA-URCHIN EGGS; MOLECULAR CHARACTERIZATION; RYANODINE RECEPTOR; CALCIUM RELEASE; CADP-RIBOSE; EXCITABILITY; MODULATION

Citation

PLOS ONE, v.6, no.10

ISSN
1932-6203
DOI
10.1371/journal.pone.0026625
URI
http://hdl.handle.net/10203/201671
Appears in Collection
BS-Journal Papers(저널논문)
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