Acoustothermal heating of polydimethylsiloxane microfluidic system

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We report an observation of rapid (exceeding 2,000 K/s) heating of polydimethylsiloxane (PDMS), one of the most popular microchannel materials, under cyclic loadings at high (similar to MHz) frequencies. A microheater was developed based on the finding. The heating mechanism utilized vibration damping in PDMS induced by sound waves that were generated and precisely controlled using a conventional surface acoustic wave (SAW) microfluidic system. The refraction of SAW into the PDMS microchip, called the leaky SAW, takes a form of bulk wave and rapidly heats the microchannels in a volumetric manner. The penetration depths were measured to range from 210 mu m to 1290 mu m, enough to cover most sizes of microchannels. The energy conversion efficiency was SAW frequency-dependent and measured to be the highest at around 30 MHz. Independent actuation of each interdigital transducer (IDT) enabled independent manipulation of SAWs, permitting spatiotemporal control of temperature on the microchip. All the advantages of this microheater facilitated a two-step continuous flow polymerase chain reaction (CFPCR) to achieve the billion-fold amplification of a 134 bp DNA amplicon in less than 3 min.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2015-07
Language
English
Article Type
Article
Keywords

POLYMERASE-CHAIN-REACTION; ACOUSTIC-WAVE MICROFLUIDICS; DNA AMPLIFICATION; PCR; DEVICES; CHIP

Citation

SCIENTIFIC REPORTS, v.5

ISSN
2045-2322
DOI
10.1038/srep11851
URI
http://hdl.handle.net/10203/200890
Appears in Collection
ME-Journal Papers(저널논문)
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