Intense acoustic load is generated when a launch vehicle lifts off, causing the damaging vibrations at the launch vehicle or satellite within the fairing. This paper is concerned with the prediction of lift-off acoustic loads for a launch vehicle. As a test example, the lift-off acoustic load on the Korean launch vehicle, NARO, is predicted by the existing calculation tool, the modified Eldred's second method. Although the acoustic sources, assumed as point sources, are to be located along the center line of the exhaust plume when using the Eldred's prediction method, the exact location of the deflected center line of exhaust gas flow is not usually known. To search for the most appropriate source positions, six models of source line distribution are suggested and the acoustic load prediction results from these models are compared with the actual measurements. It is found that the predicted sound pressure spectrum of the Naro is the most similar to the measured data when the centerline of the turbulent kinetic energy contour is used as the source line.