Hydrothermal-acid treatment for effectual extraction of eicosapentaenoic acid (EPA)-abundant lipids from Nannochloropsis salina

Hydrothermal acid treatment, was adopted to extract eicosapentaenoic acid (EPA) from wet biomass of Nannochloropsis salina. It was found that sulfuric acid-based treatment increased EPA yield from 11.8 to 58.1 mg/g cell in a way that was nearly proportional to its concentration. Nitric acid exhibited the same pattern at low concentrations, but unlike sulfuric acid its effectiveness unexpectedly dropped from 0.5% to 2.0%. The optimal and minimal conditions for hydrothermal acid pretreatment were determined using a statistical approach; its maximum EPA yield (predicted: 43.69 mg/g cell; experimental: 43.93 mg/g cell) was established at a condition of 1.27% of sulfuric acid, 113.34 degrees C of temperature, and 36.71 min of reaction time. Our work demonstrated that the acid-catalyzed cell disruption, accompanied by heat, can be one potentially promising option for omega-3 fatty acids extraction.
Publisher
ELSEVIER SCI LTD
Issue Date
2015-09
Language
ENG
Keywords

ALPHA-LINOLENIC ACID; CELL DISRUPTION; FATTY-ACIDS; HETEROTROPHIC PRODUCTION; MICROALGAL BIOMASS; ETHANOL-PRODUCTION; NITRIC-ACID; RICE STRAW; PRETREATMENT; ALGAE

Citation

BIORESOURCE TECHNOLOGY, v.191, pp.1 - 6

ISSN
0960-8524
DOI
10.1016/j.biortech.2015.04.124
URI
http://hdl.handle.net/10203/200617
Appears in Collection
CE-Journal Papers(저널논문)
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