Synthesis of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) by recombinant Escherichia coli

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Several recombinant Escherichia coli strains, including XL1-Blue, JM109, HB101, and DH5 alpha harboring a stable high-copynumber plasmid pSYL105 containing the Alcaligenes eutrophus polyhydroxyalkanoate (PHA) biosynthesis genes were constructed. These recombinant strains were examined for their ability to synthesize and accumulate poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] copolymer from glucose and either propionate or valerate. All recombinant E. coil strains could synthesize the P(3HB-co-3HV) copolymer in the medium containing glucose and propionate. However, only the homopolymer poly-(3-hydroxybutyrate) [P(BHB)] was synthesized from glucose and valerate. The PHA concentration and the 3HV fraction could be increased by inducing with acetate and/or oleate. When supplemented with oleate, the 3HV fraction increased by fourfold compared with that obtained without induction. Induction with propionate resulted in lower PHA concentration due to the inhibitory effect, but an 3HV fraction of as high as 33.0% could be obtained. These results suggest that P(3HB-co-3HV) can be efficiently produced from propionate by recombinant 5. coli by inducing with acetate, propionate, or oleate. (C) 1996 John Wiley & Sons, Inc.
Publisher
JOHN WILEY SONS INC
Issue Date
1996-03
Language
English
Article Type
Article
Citation

BIOTECHNOLOGY AND BIOENGINEERING, v.49, no.5, pp.495 - 503

ISSN
0006-3592
URI
http://hdl.handle.net/10203/19913
Appears in Collection
CBE-Journal Papers(저널논문)
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