User-friendly 3D bioassays with cell-containing hydrogel modules: narrowing the gap between microfluidic bioassays and clinical end-users' needs

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Cell-containing hydrogel modules as cell-hydrogel microunits for creating a physiologically relevant 3D in vivo-like microenvironment with multiple cell types and unique extracellular matrix (ECM) compositions facilitate long-term cell maintenance and bioassays. To date, there have been many important advances in microfluidic bioassays, which incorporate hydrogel scaffolds into surface-accessible microchambers, driven by the strong demand for the application of spatiotemporally defined biochemical stimuli to construct in vivo-like conditions and perform real-time imaging of cell-matrix interactions. In keeping with the trend of fostering collaborations among biologists, clinicians, and microfluidic engineers, it is essential to create a simpler approach for coupling cell-containing hydrogel modules and an automated bioassay platform in a user-friendly format. In this article, we review recent progress in hydrogel-incorporated microfluidics for long-term cell maintenance and discuss some of the simpler and user-friendly 3D bioassay techniques combined with cell-containing hydrogel modules that can be applied to mutually beneficial collaborations with non-engineers. We anticipate that this modular and user-friendly format interfaced with existing laboratory infrastructure will help address several clinical questions in ways that extend well beyond the current 2D cell-culture systems.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2015-06
Language
English
Article Type
Article
Keywords

MAMMALIAN-CELLS; CULTURE; MICROENVIRONMENTS; MICROFIBERS; CHIPS; MODEL; ASSAY

Citation

LAB ON A CHIP, v.15, no.11, pp.2379 - 2387

ISSN
1473-0197
DOI
10.1039/c5lc00239g
URI
http://hdl.handle.net/10203/199118
Appears in Collection
BiS-Journal Papers(저널논문)
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