Fluorescence Recovery after Merging a Surfactant-Covered Droplet: A Novel Technique to Measure the Diffusion of Phospholipid Mono layers at Fluid/Fluid Interfaces

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We present a novel technique to measure diffusion coefficients of insoluble surfactant monolayers. We merge a surfactant-coated droplet with a fluorescently labeled planar monolayer. During the merging process, a monolayer on a droplet displaces the existing planar monolayer, leaving a dark area when viewed under a fluorescence microscope. We measure fractional intensities as the dyes recover, which allows diffusion coefficients to be computed. We validate this technique with the two most common phospholipid monolayers (DPPC and DOPC) and study the diffusion of their mixtures. The proposed technique has several advantages over the FRAP technique and is potentially capable of measuring the diffusion of any soluble/insoluble surfactant monolayers.
Publisher
AMER CHEMICAL SOC
Issue Date
2014-12
Language
English
Article Type
Article
Keywords

LATERAL DIFFUSION; DPPC MONOLAYERS; LUNG SURFACTANT; WATER-INTERFACE; CELL-MEMBRANES; LIPID DROPLETS; CHOLESTEROL; MOBILITY; MODEL; MICRORHEOLOGY

Citation

LANGMUIR, v.30, no.48, pp.14369 - 14374

ISSN
0743-7463
DOI
10.1021/la503219n
URI
http://hdl.handle.net/10203/198519
Appears in Collection
BiS-Journal Papers(저널논문)CBE-Journal Papers(저널논문)
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