Efficient recovery of secretory recombinant proteins from protease negative mutant Escherichia coli strains

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Osmotic shock and lysozyme/EDTA methods were used to recover secreted recombinant proteins from protease negative mutant strains of E. coli. Up to 80% of protein A-beta-lactamase fusion protein was recovered from protease negative mutants by simple osmotic shock. Fractionation by lysozyme/EDTA treatment, increased the recovery of protein A-beta-lactamase fusion protein from the mutant strain up to 93%. Mild fractionation condition allowed efficient recovery of secreted protein from protease negative mutant strains, but not from the parent strain possessing proteases.
Publisher
KLUWER ACADEMIC PUBL
Issue Date
1998-11
Language
English
Article Type
Article
Citation

BIOTECHNOLOGY TECHNIQUES, v.12, no.11, pp.815 - 818

ISSN
0951-208X
URI
http://hdl.handle.net/10203/19800
Appears in Collection
CBE-Journal Papers(저널논문)
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