An investigation of tropomyosin 4 in the regulation of invadopodia and motility in MDA-MB-231 = An investigation of tropomyosin 4 in the regulation of invadopodia and motility in MDA-MB-231

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Tropomyosins are α-helical coiled coil proteins that bind actin filaments. Four genes encode the tropomyosin family of proteins that includes about 40 members. Studies of tropomyosins revealed a functional diversity between different isoforms. TPM4 gene encodes 2 isoforms: tropomyosin 4 low molecular weight (TM4 LMW) and tropomyosin 4 high molecular weight (TM4 HMW). TM4 LMW has been reported to regulate resorptive capacity of osteoclasts. However, the role of TM4 in facilitating degradation and motility in cancer cells has not been investigated yet. In this research, I used siRNAs to TM4 LMW and TM4 HMW and performed degradation and migration assays in MDA-MB-231 breast cancer cells to study the role of both isoforms. I found that degradation is regulated by TM4 LMW, but not TM4 HMW. This is first evidence that TM4 might regulate invadopodia dynamics in tumor cells. In addition, this result might indicate that TM4 isoforms don’t possess a redundant function. Further research should focus on mechanism underlying the observed effect by TM4 LMW. In conclusion, this study contributes to our knowledge regarding invadopodia and the role of cytoskeletal proteins in the regulation of these structures.
Advisors
David M. Helfmanresearcher데이비드 헬프만
Description
한국과학기술원 : 나노과학기술학과,
Publisher
한국과학기술원
Issue Date
2013
Identifier
566242/325007  / 020118126
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 나노과학기술학과, 2013.8, [ iii, 28 p. ]

Keywords

degradation; migration; invadopodia; invadopodia; degradation; migration; tropomyosin; tropomyosin

URI
http://hdl.handle.net/10203/197598
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=566242&flag=dissertation
Appears in Collection
NT-Theses_Master(석사논문)
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