분자생물학적 기술을 이용한 질병진단시스템 개발 = Development of diagnostic system using molecular technology

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In recent years, many approaches have been developed to detect biomolecules for diagnosis for various diseases. The success of these approaches largely relies on the integration of interdisciplinary knowledge of molecular biology, chemistry and physics. The detection system in molecular diagnostics demands high sensitivity and specificity by employing various materials and platforms such as microarray-based techniques and nanomaterials-based biosensor. Additionally, as all diagnostic devices and products are trending toward high throughput screening and miniaturization, electrical approaches as well as optic approaches come into the spotlight and its studies are vigorously progressed worldwide. This thesis introduces the development of various diagnostic tools using molecular technology for clinical disease. The value and practicality of these diagnostic systems developed in this thesis is enhanced by applying the clinical samples. First, DNA microarray for microbial pathogen detection was developed by considering high prevalence rate and/or difficulty of cultivation of pathogen. I directly determined the sequences of 23S ribosomal DNA (rDNA) and 16S-23S rDNA intergenic spacer region (ISR) for 19 bacteria with the limited or no availability of their sequences in public databases,facilitating the advance of various assays for pathogen detection. Employing bacteria-universal probes as well as species-specific probes in DNA microarray system enables to reduce false negative results by detecting all bacteria. As a result of the validation using 515 clinical isolates, the DNA microarray allowed efficient detection of bacterial pathogens with the specificities of 100% with sensitivities of 100% as well except for two pathogens. Furthermore, the detection of pathogen using DNA microarray was expanded up to 52 bloodborne pathogen, so far the largest number of pathogens. The microarrays which were applied to 112 blood culture positive samples, showed also successful i...
Advisors
Lee, Sang-Yupresearcher이상엽
Description
한국과학기술원 : 생명화학공학과,
Publisher
한국과학기술원
Issue Date
2009
Identifier
586358/325007  / 020055857
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명화학공학과, 2009.2, [ x, 132 p. ]

Keywords

DNA microarray; 감염미생물동정; 탄소나노튜브 기반 전기전도도센서; S1뉴클리아제 효소기반반응; 효소중합연쇄반응; DNA 고집적 칩; Polymerase chain reaction; S1 nuclease-based assay; Single walled carbon nanotube-based electrical sensor; Microbial pathogen detection

URI
http://hdl.handle.net/10203/196393
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=586358&flag=dissertation
Appears in Collection
CBE-Theses_Ph.D.(박사논문)
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