Effective manufacturing methods of various ginsenosides, including Rg3, Rh2 and studies on anti-cancer effects against various cancer cells using in vitro and in vivo tests = Rg3, Rh2 등을 포함한 다양한 Ginsenosides의 효율적 합성과 이들의 in vitro와 in vivo 실험을 통한 다양한 암세포에서의 항암효과 연구

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Although the biological effects of various ginsenosides, such as Rb1, Rg3, Rh2, and compound-K, have been examined, there is almost no commercial product of single or combined ginsenosides that has been developed for limited therapeutic uses. This is because of the difficulty in preparing the main ingredient (i.e., dried ginseng composed of approximately 2% of the ginsenosides, from various species), and the insufficient efficacy of each ginsenoside.Despite the large number of studies that have been conducted on a variety of ginsenosides, sufficient phar-macological activity has not been demonstrated with a single ginsenoside. Thus, the approach described here may provide evidence of the efficacy of ginensoside-based medicines. Ginsenosides with protopanaxadiol and protopanaxatriol groups, ginseng or red ginseng, which have very low levels of Rg3 and Rh2 and various phar-macological activities, are desired for efficient mass production. This study confirms that ginsenosides have potential to be used as an anticancer substance through in vitro and in vivo test of various cancer cells, especially lung cancer cells. This study also reveals effective and creative mass-manufacturing methods of rare ginsenosides that show meaningful medicinal effects and sets up various methods of manufacturing, separation, refining and analyses of ginsenosides to verify anticancer effects. The study set up solvent systems and analyzing conditions which can analyze twenty-five standard samples of gin-senosides through minimum legibile HPLC at one time. The first step for mass production of effective ginseno-sides Rg3 and Rh2 was to extract and refine the ginsenosides mixtures (GM1) from ginseng, then Rb1(351 mg/g) and Rd(86mg/g) were verified among the ginsenosides forming the mixture. The second step was to obtain GM2 containing Rg3(241 mg/g) and Rh1(173 mg/g) after acid hydrolysis and the refinement of GM1. The third step was to obtain GM3 containing F1(161 mg/g) and F2(30.5 mg/g...
Advisors
Yang, Ji-Wonresearcher양지원
Description
한국과학기술원 : 생명화학공학과,
Publisher
한국과학기술원
Issue Date
2013
Identifier
565545/325007  / 020015301
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명화학공학과, 2013.8, [ viii, 70 p. ]

Keywords

ginsenoside; 알에이치투; 알지쓰리; 폐암; 항암; 진세노사이드; anti-cancer; lung cancer; Rg3; Rh2

URI
http://hdl.handle.net/10203/196356
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=565545&flag=dissertation
Appears in Collection
CBE-Theses_Ph.D.(박사논문)
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